Introduction：

The focal point of the objective lens in a microscope is reformed into a “conjugate” focal plane close to the detection system. By inserting an aperture diaphragm (Pinhole) in this conjugate focal plane, we can obstruct fluorescence emission from planes OUTSIDE of the primary focus point.

• Point source laser is scanned in raster pattern over sample using Galvanometric mirrors

• Galvo Mirrors move beam in X and Y dimensions for EVERY pixel in the digital image

• Intensity values (within a 12 bit dynamic range) are recorded by PMT for every pixel

Function（application）：

•Diode laser 405nm； 488nm； 555nm ；639nm

•Plan-Apochromat 5x,NA0.16；Plan-Apochromat 10x,NA0.45；Plan-Apochromat 20x,NA0.8；Plan-Apochromat 40x,NA0.95；Plan-Apochromat 63x,NA1.4

Experimental Items：

• Multi-fluorescent specimens with distinguishable three dimensionality

• Samples that require localized exposure

• Photobleaching Experiments (FRAP)

• Time series Experiments

• Samples with overlapping probes (META)