儀器功能 / Instrument Functions
儀器簡介 / Instrument Introduction
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將待測物(通常為蛋白質或胜肽)與介質混合,介質吸收雷射光後將一個或多個質子轉移給待測物,使其離子化,進入 TOF Mass Analyzer 分析。
The sample (typically proteins or peptides) is mixed with a matrix. The matrix absorbs laser energy and transfers one or more protons to the analyte, ionizing it for analysis by the TOF mass analyzer. -
技術類型 / Model & Ionization Source:
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MALDI-TOF(Matrix-Assisted Laser Desorption/Ionization Time-of-Flight)
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分析功能 / Analytical Functions
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蛋白質分子量測定 / Protein Molecular Weight Determination
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聚合物分子量測定 / Polymer Molecular Weight Determination
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蛋白質水解後指紋比對 / Protein Digestion Fingerprint Comparison
規格 / Specifications
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質量分析模式 / Mass Analyzer Modes:
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Linear TOF:解析度 > 5,000 m/Δm;準確度 < 20 ppm
Linear Time-of-Flight: Resolution > 5,000 m/Δm; Accuracy < 20 ppm -
Reflectron TOF:解析度 > 20,000 m/Δm;準確度 < 5 ppm
Reflectron Time-of-Flight: Resolution > 20,000 m/Δm; Accuracy < 5 ppm
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靈敏度 / Sensitivity:500 amol
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最大取樣速率 / Maximum Sampling Rate:200 Hz
儀器放置地點 / Location
- 國際學術研究大樓 八樓 IR805室 - 質譜儀室
8th Floor, IR805 Room, International Research Building – Mass Spectrometry Laboratory
申請流程 & 注意事項 / Application & Notes
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聯繫管理師資 / Contact Instructor
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請先聯繫管理師資 呂濟宇副教授 (2137#499),討論樣品屬性及約定教學時段。
Contact the supervising instructor, Prof. Chi-Yu Lu (2137#499), to discuss sample properties and schedule training sessions
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教學與認證 / Training & Certification
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經管理師資教學與認證後,方可自行操作儀器。
After receiving training and certification from the instructor, users may operate the instrument independently.
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繳費與測定 / Payment & Measurement
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每次測定樣品前,請填妥繳費單,經繳費後方可進入質譜儀室使用。
Complete the payment slip before each sample measurement; access to the mass spectrometer room is allowed only after payment.
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使用後檢核 / Post-Use Check
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使用完畢後,請務必檢核雷射托盤是否歸位。
After use, ensure the laser target plate is properly returned.
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注意事項 / Notes
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送測樣品不可含任何形式之鹽類(緩衝液需自行移除)。
Samples must not contain any form of salts (buffers should be removed by the user). -
本中心僅提供 α-Cyano-4-Hydroxycinnamic Acid (CHCA) 及 Sinapinic Acid (SA) 兩種基質,其餘基質需自行準備。
The center provides only CHCA and Sinapinic Acid (SA) matrices; users must supply any other matrices themselves.
📄 下載申請表 / Download Form
使用收費標準 / Fees
校內使用者 / Internal Users:
自行測定:雷射托盤計收,不計點數100元/盤。每月不計使用量,為500元/月。
委託測樣:雷射托盤計收,150元/點。
校外使用者 / External Users:
委託測樣:雷射托盤計收,1500元/點。
育成中心進駐廠商 / Incubation Center Tenant:
委託測樣:雷射托盤計收,不計點數400元/盤。每月不計使用量,為2,000元/月。

