儀器功能 / Instrument Functions

儀器簡介 | Instrument Introduction

  • 本儀器為超高解析質譜儀 Impact HD,具雙質譜系統:前段為 四極桿質譜 (Quadrupole MS),後段為 飛行時間質譜 (TOF MS)
    The instrument is a high-resolution mass spectrometer, Impact HD, featuring a dual mass analyzer: the first stage is a Quadrupole MS, and the second stage is a TOF MS.

  • 同時支援 MSMS/MS 模式,具備高解析度(40,000 FWHM)與高質量準確度(<2 ppm)。
    It supports both MS and MS/MS modes, providing high resolution (40,000 FWHM) and high mass accuracy (<2 ppm).

  • 搭配 超高效能液相層析系統 (UHPLC)極致效能液相層析系統 (nanoLC),可進行 LC–MS/MS 分析。
    Equipped with UHPLC and nanoLC systems, enabling LC–MS/MS analysis.

測定項目 | Applications

  • 蛋白質身份鑑定 / Protein Identification:提供 .mgf 檔,可使用 MASCOT 軟體比對。
    Provides .mgf files for protein identification, which can be analyzed using MASCOT software.

  • 蛋白質與胜肽分子量測定 / Protein and Peptide Mass Determination
    Determination of protein and peptide molecular masses.

  • 未知物質定性分析 / Unknown Compound Identification:使用者須自備分析資料庫以進行比對。
    Qualitative analysis of unknown compounds; users must provide their own database for comparison.

儀器放置地點 / Location

  • 國際學術研究大樓 八樓 IR805室 - 質譜儀室
    8th Floor, IR805 Room, International Research Building – Mass Spectrometry Laboratory

申請流程 & 注意事項 / Application & Notes

  1. 請至國科會基礎研究核心設施預約服務管理系統預約儀器 / Reserve via NSTC Core Facilities System
  2. 送測樣品前,請先致電與儀器操作技術人員討論樣品屬性與溶劑等初步測試條件。
    Before submitting samples, contact the instrument technician to discuss sample properties and solvent/initial testing conditions.
  3. 確定進行送檢測試後,請填妥繳費單,至本校出納組繳費,並將繳費單與待檢樣品交予本中心,本中心將安排測試時間。
    Once confirmed, complete the payment slip, pay at the Cashier Office, and submit the payment slip along with the sample to the center. The center will schedule the analysis.

注意事項 / Notes

  • 儀器以操作技術人員代為操作為主,以維護儀器正常運作。
    Instrument operation is primarily handled by the technician to ensure proper functioning.

  • 樣品可為固態、液態或溶液;溶液濃度需 ≥1 ppm 或 ≥1 pmole/μl。
    Samples can be solid, liquid, or solution; solution concentration must be ≥1 ppm or ≥1 pmole/μl.

  • 樣品需可溶於水、甲醇或乙腈 (Acetonitrile),並註明所用溶劑。
    Samples must be soluble in water, methanol, or acetonitrile, and the solvent should be specified.

  • 禁止含有放射性、腐蝕性、強酸鹼、界面活性劑或磷酸鹽等非揮發性鹽類的樣品。
    Samples must not contain radioactive, corrosive, strong acids/bases, surfactants, or non-volatile salts such as phosphates.

  • 依科技部要求撰寫「貴儀年度工作報告」,使用儀器之實驗結果若發表論文,請於致謝 (Acknowledgement) 處提及本中心。
    Per MOST requirements for the annual core facility report, please acknowledge the facility in publications that use the instrument.

📄 下載申請表 / Download Form

使用收費標準 / Fees

校內使用者 / Internal Users:

蛋白質鑑定: 600元件;蛋白質及胜肽分子量測定:400元/件

校外使用者 / External Users: 

蛋白質鑑定: 3,600元件;蛋白質及胜肽分子量測定:1,200元/件

育成中心進駐廠商 / Incubation Center Tenant:

蛋白質鑑定: 2,400元件;蛋白質及胜肽分子量測定:800元/件

💳 詳細收費表 / Fee Table

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