儀器功能 / Instrument Functions
儀器簡介 | Instrument Introduction
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本儀器為超高解析質譜儀 Impact HD,具雙質譜系統:前段為 四極桿質譜 (Quadrupole MS),後段為 飛行時間質譜 (TOF MS)。
The instrument is a high-resolution mass spectrometer, Impact HD, featuring a dual mass analyzer: the first stage is a Quadrupole MS, and the second stage is a TOF MS. -
同時支援 MS 與 MS/MS 模式,具備高解析度(40,000 FWHM)與高質量準確度(<2 ppm)。
It supports both MS and MS/MS modes, providing high resolution (40,000 FWHM) and high mass accuracy (<2 ppm). -
搭配 超高效能液相層析系統 (UHPLC) 與 極致效能液相層析系統 (nanoLC),可進行 LC–MS/MS 分析。
Equipped with UHPLC and nanoLC systems, enabling LC–MS/MS analysis.
測定項目 | Applications
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蛋白質身份鑑定 / Protein Identification:提供 .mgf 檔,可使用 MASCOT 軟體比對。
Provides .mgf files for protein identification, which can be analyzed using MASCOT software. -
蛋白質與胜肽分子量測定 / Protein and Peptide Mass Determination。
Determination of protein and peptide molecular masses. -
未知物質定性分析 / Unknown Compound Identification:使用者須自備分析資料庫以進行比對。
Qualitative analysis of unknown compounds; users must provide their own database for comparison.
儀器放置地點 / Location
- 國際學術研究大樓 八樓 IR805室 - 質譜儀室
8th Floor, IR805 Room, International Research Building – Mass Spectrometry Laboratory
申請流程 & 注意事項 / Application & Notes
- 請至國科會基礎研究核心設施預約服務管理系統預約儀器 / Reserve via NSTC Core Facilities System
- 送測樣品前,請先致電與儀器操作技術人員討論樣品屬性與溶劑等初步測試條件。
Before submitting samples, contact the instrument technician to discuss sample properties and solvent/initial testing conditions. - 確定進行送檢測試後,請填妥繳費單,至本校出納組繳費,並將繳費單與待檢樣品交予本中心,本中心將安排測試時間。
Once confirmed, complete the payment slip, pay at the Cashier Office, and submit the payment slip along with the sample to the center. The center will schedule the analysis.
注意事項 / Notes
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儀器以操作技術人員代為操作為主,以維護儀器正常運作。
Instrument operation is primarily handled by the technician to ensure proper functioning. -
樣品可為固態、液態或溶液;溶液濃度需 ≥1 ppm 或 ≥1 pmole/μl。
Samples can be solid, liquid, or solution; solution concentration must be ≥1 ppm or ≥1 pmole/μl. -
樣品需可溶於水、甲醇或乙腈 (Acetonitrile),並註明所用溶劑。
Samples must be soluble in water, methanol, or acetonitrile, and the solvent should be specified. -
禁止含有放射性、腐蝕性、強酸鹼、界面活性劑或磷酸鹽等非揮發性鹽類的樣品。
Samples must not contain radioactive, corrosive, strong acids/bases, surfactants, or non-volatile salts such as phosphates. -
依科技部要求撰寫「貴儀年度工作報告」,使用儀器之實驗結果若發表論文,請於致謝 (Acknowledgement) 處提及本中心。
Per MOST requirements for the annual core facility report, please acknowledge the facility in publications that use the instrument.
📄 下載申請表 / Download Form
使用收費標準 / Fees
校內使用者 / Internal Users:
蛋白質鑑定: 600元件;蛋白質及胜肽分子量測定:400元/件
校外使用者 / External Users:
蛋白質鑑定: 3,600元件;蛋白質及胜肽分子量測定:1,200元/件
育成中心進駐廠商 / Incubation Center Tenant:
蛋白質鑑定: 2,400元件;蛋白質及胜肽分子量測定:800元/件

