儀器簡介 / Introduction

• 數位化聚合酶反應分析系統是一台精準核酸定量分析儀，利用微滴化技術將樣品分散成數萬液滴，再透過 PCR 放大進行終點檢測與分子計數，實現核酸分子絕對定量。
• The digital PCR system is a precise nucleic acid quantification instrument. It partitions samples into tens of thousands of droplets and uses PCR amplification for endpoint detection and molecular counting, enabling absolute quantification of nucleic acids.

儀器特點 / Features

• 微滴化技術降低 PCR 抑制物或背景影響，並減少聚合酶偏差。 / Droplet partitioning reduces PCR inhibitors and background effects, minimizing polymerase bias.
• 精確放大目標片段，提高定量精度與靈敏度，優於 real-time PCR。 / Precise amplification in micro-reaction wells improves accuracy and sensitivity, superior to real-time PCR.
• 最低偵測靈敏度 0.001%。 / Detection sensitivity down to 0.001%.
• 可同時讀取 FAM 與 VIC 螢光訊號。 / Dual-channel detection for FAM and VIC fluorescence.
• 無需標準品即可進行基因絕對定量。 / Absolute quantification without the need for standards.
• 全自動分析 96 個樣品一次完成。 / Fully automated analysis of up to 96 samples per run.

功能應用 / Applications

• 基因絕對定量分析 / Absolute quantification of genes
• 基因拷貝數 (copy number) 變異分析 / Gene copy number variation analysis
• 基因點突變檢測 / Detection of point mutations
• 適用於 NGS 前端樣品製備或後端資料確認 / Suitable for NGS sample preparation and downstream data validation

儀器放置地點 / Location

• 國際學術研究大樓八樓 IR801 Real-Time PCR室
  8th Floor, International Academic Research Building, Room IR801 – Real-Time PCR Laboratory