儀器功能 / Instrument Functions
儀器簡介 / Instrument Introduction
- 流式細胞術(Flow Cytometry)是學術與臨床上不可或缺的技術。FACSMelody 細胞分選儀可簡化操作流程,提高工作效率,並提供自動分選能力,減少人工操作時間。。
Suspended cells are labeled with fluorescent antibodies and analyzed using a flow cytometer with laser excitation to identify cell types and characteristics.The BD FACSMelody cell sorter streamlines workflow, minimizes hands-on time, and enables walk-away sorting.
應用功能 / Applications & Functions
-
細胞純化 / Cell Purification
純化目標細胞族群進行後續培養、定序等研究,最多可同時分選四種細胞族群。 / Purify target cell populations for downstream culture, sequencing, or other studies; can sort up to 4 populations simultaneously. -
單細胞分析 / Single-Cell Analysis
可將單顆細胞收集在 96 孔盤中進行單細胞 RNA 提取與定序。透過索引分選,可將基因表達與單細胞蛋白質表達關聯。 / Collect single cells into 96-well plates for single-cell RNA extraction and sequencing; index sorting links gene and protein expression. -
外泌體分析 / Extracellular Vesicle Analysis
搭配外泌體捕獲套組,可輕鬆分離外泌體進行研究。 / Facilitates extracellular vesicle isolation using capture kits.
儀器規格 / Specifications
-
搭載 3 支雷射(405 nm、488 nm、640 nm),可偵測 9 種螢光通道。 / 3 lasers (405 nm, 488 nm, 640 nm) with 9 fluorescence channels.
-
支援 4 向分選或盤式分選。 / 4-way sorting or plate sorting capability.
-
全自動螢光補償。 / Fully automated fluorescence compensation.
-
分選純度 >98%,回收率 >80%。 / Sorting purity >98%, recovery >80%.
-
自動化品管系統,確保數據一致性。 / Automated QC system maintains experimental data consistency.
儀器放置地點 / Location
- 國際學術研究大樓 八樓 IR808室-生物醫學影像核心研究室
International Academic Research Building, 8F, Room IR808 – Biomedical Imaging Core Facility
申請流程 & 注意事項 / Application & Notes
-
下載螢光配色參考表作為設計依據。 / Download the fluorescence panel reference sheet for experiment design.
-
自行上機 / Independent Use
每半年舉辦一次教育訓練課程,通過後取得認證,再向技術員申請帳號,即可上網預約儀器。
Attend the semi-annual training course; after certification, request an account from the technician to book instrument online. -
委託上機 / Assisted Use
需提前一週與技術員預約上機時間(實驗人員陪同),並討論檢體製備。
Schedule assisted use at least one week in advance with a technician and discuss sample preparation. -
每月依實際使用時數開立繳費單,使用者依單據繳納。
Monthly billing is based on actual usage; pay according to the invoice.
注意事項 / Notes
-
本機含 3 根雷射(405/488/640 nm),可激發 9 種螢光,請依網站參考表挑選螢光組合;檢體製備請參照注意事項。
The instrument has 3 lasers (405/488/640 nm) and 9 fluorescence channels; select appropriate combinations per the reference sheet. Follow sample preparation guidelines. -
自行上機者需自行準備流式細胞校正磁珠(BD FACS Accudrop Beads, BD#661612),或洽中心提供(耗材費另計)。
Independent users must provide calibration beads (BD FACS Accudrop Beads, BD#661612) or request from the center (additional cost). -
禁用隨身碟,請使用空白光碟燒錄資料。電腦硬碟空間有限,資料將不定期刪除,中心不負保管責任。
USB drives are prohibited; use blank CDs to save data. Center is not responsible for stored data. -
預約時段請準時上機,遲到超過 30 分鐘或未到者視同放棄。
Arrive on time for booked slots; no-shows or >30 min late will forfeit the session. -
預約後如不使用,請於使用前一天取消;若兩次未取消,停權一個月。
Cancel at least 1 day in advance; two no-shows will suspend booking privileges for 1 month. -
使用中如發現異常立即停止並聯絡技術員,因操作不當造成損壞,維修費用由使用者及所屬實驗室負責。
Stop immediately and contact a technician if issues arise; repair costs due to misuse are the responsibility of the user and their lab.
📄 下載申請表 / Download Form
使用收費標準 / Fees
校內使用者 / Internal Users:
自行操作: 1,800元/3小時
委託操作: 2,400元/3小時+開機費1,000元/次
校外使用者 / External Users:
委託操作: 4,800元/3小時+開機費2,000元/次
育成中心進駐廠商 / Incubation Center Tenant:

