應用與功能 / Applications and Functionality

• 分選後細胞可直接用於培養、移植、核酸提取、單細胞PCR或原位雜交
  Sorted cells can be used directly for culture, transplantation, nucleic acid extraction, single-cell PCR, or in situ hybridization

• 適合研究細胞基因、蛋白質、功能特性及不同細胞間差異
  Suitable for studying cellular genes, proteins, functional characteristics, and intercellular differences

• 高回收率與存活率，特別適用於幹細胞及極低含量細胞研究
  High recovery and viability, ideal for stem cells and rare cell populations

• 支援單一細胞分選，便於挑選穩定細胞株或進行單細胞NGS定序
  Supports single-cell sorting, useful for selecting stable clones and single-cell NGS sequencing

雷射與螢光檢測系統 / Lasers and Fluorescence Detection

• 配備三支固態雷射：488 nm、633 nm、UV nm
  Equipped with three solid-state lasers: 488 nm, 633 nm, UV nm

• 可分析七種螢光波段 / Detects seven fluorescence channels

  • 488 nm laser:

    • FL1 (530/40 nm BP) for FITC, GFP

    • FL2 (580/30 nm BP) for PE, YFP

    • FL3 (613/20 nm BP) for PE-TexRed, PI (DNA)

    • FL4 (670/30 nm BP) for PE-Cy5, 7-AAD (DNA)

  • UV laser:

    • FL5 (457/50 nm BP) for DAPI or Hoechst blue

    • FL6 (628/32 nm BP) for Hoechst red

  • 633 nm laser:

    • FL7 (670/30 nm BP) for APC

分選性能與操作規格 / Sorting Performance and Operational Specs

• 溫控系統：4–40℃，提高細胞活性
  Temperature control: 4–40°C to maintain cell viability

• 上樣樣品管：1.5, 5, 50 mL tubes
  Sample tube compatibility: 1.5, 5, 50 mL

• 噴嘴大小：70 μm
  Nozzle size: 70 μm

• 分選格式：6–1538 microplate 或 4-way sort (1.5, 5, 15, 50 mL tubes)
  Sorting format: 6–1538 microplate or 4-way sort (1.5, 5, 15, 50 mL tubes)

• 分析速度：100,000 events/sec
  Analysis speed: 100,000 events/sec

• 分選速度：回收率>90%，純度>99% 時可達 70,000 events/sec
  Sorting speed: up to 70,000 events/sec at >90% recovery and >99% purity

• 分選模式：Enrich / Purity / Single
  Sorting modes: Enrich, Purity, Single

儀器放置地點 / Location

• 國際學術研究大樓12樓貴重儀器室
• Precious Instrument Room, 12F, International Academic Research Building