儀器功能 / Instrument Functions

儀器簡介 / Instrument Introduction

  • Epoch 2 微量盤分光光度計為全波長吸收光偵測系統，可支援 6 至 384 孔微量盤、比色管及微量樣本檢測，結合 Gen5 軟體可執行樣品偵測、數據分析與結果報告。其光學系統具備 200–999 nm 的全波長偵測範圍與高達 0.0001 OD 的解析度，能進行精確的吸光值測定。儀器支援線性（Linear）與迴轉式（Orbital）震盪功能，可依實驗需求進行樣品混合與反應均化，適用於多種生物化學與分子生物學分析。搭配 BioTek Take 3 微量偵測盤，可使用極微量樣本進行多樣分析，每次可同時測定 16 個樣品，樣本體積僅需 2 µL，並具備獨立光徑校正功能，能有效提升微量定量的精確度與重現性。

    The Epoch 2 Microplate Spectrophotometer is a full-spectrum absorbance detection system compatible with 6–384 well microplates, cuvettes, and micro-volume samples. Combined with the Gen5 software, it performs detection, data analysis, and report generation with ease. The optical system covers a full wavelength range from 200 to 999 nm with a high absorbance resolution of 0.0001 OD, ensuring precise quantification. The instrument supports both linear and orbital shaking modes for optimal sample mixing and reaction uniformity, making it suitable for a wide range of biochemical and molecular biology applications. When used with the BioTek Take 3 Micro-Volume Plate, the system enables simultaneous measurement of up to 16 samples with as little as 2 µL per sample, featuring individual optical path calibration for enhanced accuracy and reproducibility in micro-volume quantification.

服務目的 / Purpose of Service

  • 提供共用性高及貴重之儀器，使各方面之醫學研究領域得以順利發展，並能利用尖端之實驗技術及精密之儀器設備提昇研究品質。 / To provide highly shared and valuable instruments to facilitate the development of various medical research fields, enabling the use of advanced experimental technologies and precision equipment to enhance research quality.

應用功能 / Applications

  • Epoch 2 結合 Take 3 微量偵測盤可滿足多樣化實驗需求，特別適用於核酸、蛋白質及酵素反應等吸光度分析。/ The Epoch 2 with Take 3 Micro-Volume Plate supports a broad spectrum of assays requiring absorbance-based measurements, ideal for nucleic acid, protein, and enzyme activity studies.
    主要應用包含：/ Key applications include:

    • ELISA 與微量盤吸光度分析（免疫定量檢測）/ ELISA and microplate absorbance assays (immunoassays)

    • 酵素動力學分析（Enzyme Kinetics）/ Enzyme kinetics and reaction monitoring

    • 微生物生長曲線監測與比色分析 / Microbial growth curve and optical density analysis

    • 核酸（DNA/RNA）與蛋白質定量分析 / DNA/RNA and protein quantification

    • 低體積樣本（Micro-volume）快速偵測與比較實驗 / Micro-volume sample detection and comparative analysis

儀器放置地點 / Location

  • 國際學術研究大樓（國研大樓）10 樓 共同實驗室
    Shared Laboratory, 10th Floor, International Academic Research Building

申請流程 & 注意事項 / Application & Notes

  • 於實驗室操作貴重儀器需先受講習與認證 / Operation of any valuable instruments requires prior training and certification.

  • 需登記使用紀錄的儀器設備請確實登記 / Instruments requiring usage registration must be properly recorded.

  • 使用完畢請隨手關閉電源 / Always turn off equipment after use.

  • 廢棄物請依性質分類、減量與處理 / Waste must be sorted, minimized, and properly disposed.

  • 最後一位使用者離開時務必關閉所有燈源 / The last user leaving must turn off all lights.

  • 實驗室內禁止喧鬧、佔用或翻動他人物品 / No loud noise, occupying space, or moving others’ items without permission.

  • 公共空間或設備上的物品需標示使用者、單位及聯絡方式 / Items in public spaces or equipment must be labeled with user, unit, and contact information.

  • 使用後務必維護空間與設備清潔並復原原狀 / Maintain cleanliness and return space/equipment to original condition after use.

  • 有毒物質必須在 fume hood 中操作 / Toxic substances must be handled inside a fume hood.

  • 進入實驗室前需了解可能危險與安全防護措施 / Understand potential hazards and required safety measures before any lab activity.

  • 所有化學品應清楚標示 / All chemicals must be clearly labeled.

  • 熟悉緊急應變步驟及設備位置（如滅火器、逃生呼吸器等） / Be familiar with emergency procedures and locations of emergency equipment (e.g., fire extinguishers, escape respirators).

  • 暫存資料於儀器附屬電腦需放 D 槽指定資料夾，並盡可能當天以 CD 燒錄帶走 / Temporary data on instrument computers should be saved in D drive teacher folders and, if possible, burned to CD the same day.

  • 遇問題或異常情況請告知管理員或負責老師 / Report any issues or abnormal situations to the lab manager or responsible faculty.

注意事項 / Notes

  • 感染性液體廢棄物：高壓滅菌或 5–10% 漂白水滅菌。/ Infectious liquid waste: Sterilize by autoclaving or with 5–10% bleach.

  • 非感染性液體廢棄物：依一般廢液處理。/ Non-infectious liquid waste: Dispose according to standard liquid waste procedures.
    養菌菌液、Agar plate 等物品，請包紮妥當並經滅菌才可丟棄。Culture broths, agar plates, and similar items must be properly packaged and sterilized before disposal.
    EB-stained gel 需經袋子包紮妥當，才可丟入垃圾桶。EB-stained gels must be securely bagged before disposal in the trash.

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