儀器概述 / Instrument Overview

• ONT PromethION 2 Solo 為 Oxford Nanopore Technologies（ONT）所開發之高通量奈米孔定序平台，採用奈米孔電流變化原理進行 DNA 與 RNA 序列判讀。該系統具備長讀長、即時數據輸出與高度彈性的實驗配置能力，特別適用於需要解析複雜基因組結構或長片段資訊的研究應用。PromethION 2 Solo 採模組化 Flow Cell 設計，可同時執行多組樣本，兼顧高產能與操作彈性。
  The ONT PromethION 2 Solo is a high-throughput nanopore sequencing platform developed by Oxford Nanopore Technologies (ONT). It utilizes changes in ionic current across nanopores to directly read DNA and RNA sequences. Featuring ultra-long read lengths, real-time data generation, and flexible experimental configurations, the system is well suited for studies requiring comprehensive characterization of complex genomes or long-range sequence information. Its modular flow cell design allows simultaneous processing of multiple samples with high scalability.

功能與應用 / Functions & Applications

• 長片段基因體定序：可產生超過 100 kb 甚至 Mb 級讀長，有利於高品質基因組組裝與重複序列解析。
  Long-read genome sequencing: Generates reads exceeding 100 kb and even megabase-scale lengths, enabling high-quality genome assembly and resolution of repetitive regions.

• 結構變異分析（SVs）：有效鑑定大型插入、缺失、倒位、易位及拷貝數變異等複雜基因組變化。
  Structural variant analysis (SVs): Accurately detects large insertions, deletions, inversions, translocations, and copy number variations.

• 轉錄體與 RNA 定序：支援 Direct RNA Sequencing，無需反轉錄即可直接讀取 RNA 序列，保留完整轉錄與修飾資訊。
  Transcriptome and RNA sequencing: Supports direct RNA sequencing without reverse transcription, preserving native RNA structures and modifications.

• 表觀遺傳分析：透過電流訊號差異直接偵測 DNA/RNA 修飾（如 5mC、6mA），無需額外化學處理。
  Epigenetic analysis: Detects DNA and RNA modifications (e.g., 5mC, 6mA) directly from electrical signals without additional chemical treatments.

• 微生物與宏基因體研究：適用於病原體鑑定、微生物多樣性分析及臨床快速檢測應用。
  Microbial and metagenomic studies: Ideal for pathogen identification, microbial diversity profiling, and rapid diagnostic applications.

分析與數據 / Analysis & Data

• 高通量與可擴充性：PromethION 2 Solo 具備高資料產出能力，可依研究需求彈性調整測序策略。
  High throughput and scalability: PromethION 2 Solo delivers high data output with flexible sequencing strategies tailored to different research needs.

• 即時數據監控：定序過程中即可同步產出與分析數據，研究人員能即時掌握品質、讀長與覆蓋度。
  Real-time data monitoring: Sequencing data are generated and analyzed in real time, allowing immediate assessment of quality, read length, and coverage.

• 長讀長優勢：有效提升 de novo genome assembly 的完整性，並解析複雜結構變異。
  Long-read advantage: Enhances de novo genome assembly quality and enables comprehensive analysis of complex genomic structures.

• 完整分析生態系：可搭配 ONT 與開源分析工具（如 Guppy、Minimap2、Flye、Medaka、NanoPlot），進行序列比對、組裝、錯誤校正與品質評估。。
  Integrated bioinformatics ecosystem: Compatible with ONT and open-source tools (e.g., Guppy, Minimap2, Flye, Medaka, NanoPlot) for alignment, assembly, polishing, and quality control..

儀器放置地點 / Location

• 國際學術研究大樓 八樓 IR802室 - 蛋白質體核心研究室
  International Research Building, 8th Floor, Room IR802 – Proteomics Core Laboratory