儀器預設程序將機械解離與酶消化過程結合,無須專人看守,
全自動化完成組織樣品的解離。獲得的單細胞保持良好活性,
同時盡可能完整保留各個抗原表位,使得下游分析更加可靠。
相較傳統手動解離方式可快速地完成樣品製備過程。
研發人員開發之預設程序配合標準解離試劑盒操作步驟保證實驗結果重複性佳儀器搭配一次性解離管為全封閉設計,
一方面保護樣品不受汙染 ;另一方面,當處理有毒或感染性樣品可保護操作者安全,
可製備組織樣品單細胞懸液並應用於下游細胞分離、分析及培養;
亦可用於組織樣品匀漿與分離細胞器、提取核酸或是蛋白質,
可快速全自動的獲得單細胞懸液同時保護細胞活性,
且擁有八個通道可獨立操作運用於不同組織的解離,當某個通道程序進行完成,
亦可隨時加入新的樣本繼續下一程序。
Introduction
The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. The instrument offers optimized gentleMACS Programs for a variety of specific applications.
Function(application):
Optimized, pre-set gentleMACS Programs have been developed for easy dissociation of tissues into single-cell suspensions, including:mouse or human tumor、mouse or rat neonatal heart、neural tissue、mouse spleen, lung, lamina propria, muscle, epidermis, or liver、mouse or human skinand more
Also, programs have been developed for the homogenization of tissues, for: total RNA or mRNA isolation from fresh or frozen samples、protein extraction、determination of bacterial or viral load、mitochondria isolation